Analysis Note

ControlJurkat whole cell lysate, K-562 whole cell lysate

Application

Indirect immunofluorescence: 1:20-1:30. Use 30-100 µl per well/slide.

Immunohistochemistry in formalin fixed paraffin embedded tissues: requires citrate acid microwave or HIER pretreatment: 1:20-1:30 primary antibody dilution. Archival tissues will require more antibody than 24-48 fixed specimens.

Optimal working dilutions must be determined by the end user.

This Anti-Nuclear Ribonucleoprotein Antibody, clone 58-15 is validated for use in IF, IH, IH(P) for the detection of Nuclear Ribonucleoprotein.

Research Sub CategoryNeural Stem CellsRNA Binding Protein (RBP)

General description

snRNPs or small nuclear ribonucleoproteins, are particles that combine with pre-mRNA and various proteins to form spliceosomes (a type of large molecular complex). SnRNPs "recognize" the places along a strand of pre-mRNA and are essential in the removal of introns. These molecules are found within the cell′s nucleus. The two essential components of snRNPs are protein molecules and RNA. The RNA found within each snRNP particle is known as small nuclear RNA, or snRNA. These molecules are usually about 150 nucleotides long. The snRNA is bound by a Ribonuclear protein (RNP) to activate its enzymatic activity. The precise beginnings and ends of introns on the primary transcripts are marked by signals so that they can be recognized and removed. The intron-boundary signals are recognized by a short bit of RNA found in curious RNA-protein complexes known as the small nuclear ribonucleoprotein particles - snRNPs, for short. At least four different kinds of snRNPs cooperate in most splicing. The RNA in these particles is like ribosomal RNA in that it is used directly, and has both an enzymatic and a structural role.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Specificity

Stains nuclear ribonucleoprotein particles (RNP) in human cells. Does not cross react with mouse RNP, other species not tested. RNP are 20-80nm dense nuclear particles. Highest labeling densities are found in RNP, but heterochromatin, euchromatin and nucleoli are also stained.

Target description

28 kDa